Republican nominee Donald Trump has called House Speaker Paul Ryan as a 'weak and ineffective' leader.
Mr Trump added that Republicans were coming at him "from all sides" and their disloyalty was harder to fight than the Democrats.
According to BBC News, Mr Ryan is the newest Republican not to back Mr Trump following the 2005 video that surfaced showing him making obscene comments about groping women.
Nonetheless, Mr Trump has been intensely supported by running mate Mike Pence.
"You have nominated a man for president who never quits, who never backs down. He is a fighter, he is a winner," Mr Pence said at an event in Iowa, praising him for asking forgiveness during Sunday's debate for his lewd comments.
Star FM Online also said that the PRRI/Atlantic poll revealed two days after the second US presidential debate suggested Mrs Clinton holds a 49-38 lead over her opponent. This means that Mr Trump is 11 points behind his rival.
Last Friday, a video was released which shows Mr Trump describing how he had sought to have sex with a married woman and making other sexually aggressive comments about women.
As a result, President Barack Obama's spokesman said Mr Trump's remarks were 'repugnant' and amounted to sexual assault.
Around half of the 331 incumbent Republican senators, House members and governors have condemned Trump's lewd remarks on the video and about ten percent have called for him to drop out of the race.
On Monday, Mr Ryan told fellow House Republicans he would focus on congressional elections to ensure Republicans could maintain legislative control.
Mr Trump answered back in a string of tweets, stating the 'shackles' had been removed, allowing him to "fight for America the way I want to".
He also fired at Senator John McCain, who has denounced Trump's conduct and faces a close re-election battle, as 'foul-mouthed'.
Though these had happened, some Republicans are still sticking by Mr Trump's side.